Announcement | EZassay Contributes to the Standardization of Isothermal Amplification Testing

Recently, the China Inspection and Testing Society (CITS) released the group standard Technical Requirements for Pathogenic Microorganism Detection Based on Nucleic Acid Isothermal Amplification (T/CITS 520-2025).

As a principal drafting unit, Shenzhen EZassay Biotechnology Co., Ltd. actively participated in the development, technical drafting, and validation of the standard, contributing to the advancement of isothermal amplification testing toward greater standardization and methodological consistency.

The standard defines comprehensive technical requirements for nucleic acid isothermal amplification testing, including terminology and abbreviations, sample collection and processing, nucleic acid extraction, amplification methods, result interpretation, and contamination control. It provides a structured technical reference for pathogenic microorganism detection.

Isothermal amplification is a rapid detection approach well suited for frontline and field applications. Unlike conventional PCR or qPCR, isothermal amplification operates at a constant temperature, enabling rapid nucleic acid amplification without thermal cycling. Its advantages—simple operation, short turnaround time, and lower reagent cost—make it particularly suitable for primary laboratories, on-site testing, and decentralized settings. Today, isothermal amplification technologies have expanded beyond laboratory research into real-world applications, including clinical diagnostics, customs inspection and quarantine, and food and environmental safety, becoming an important tool for rapid detection.

Transforming frontline experience into standardized practice, EZassay leveraged its extensive R&D background and application experience to provide optimization recommendations throughout the standard, covering sample handling, nucleic acid extraction, isothermal amplification procedures, and result interpretation. These contributions help improve workflow consistency and enhance the reliability of detection results.

As a dedicated provider of isothermal amplification solutions, EZassay focuses on technologies including RPA, RAA, LAMP, and RCA. The company develops and manufactures a comprehensive portfolio of high-performance reagent kits and core enzyme products, supporting molecular diagnostic development across diverse application scenarios, and providing reliable technical support for research, disease detection, and public health monitoring.

Looking ahead, EZassay will continue to uphold its mission of Simplifying Science, Empowering Research. Guided by standards, the company remains committed to promoting the standardized application of isothermal amplification technologies and supporting the precise and efficient development of public health and the testing industry.