2026-8-5

LbaCas12a +57% Trans-Cleavage — Stronger Signal, Faster Readout

Higher trans-cleavage efficiency → Faster signal accumulation → Lower detection limit → Earlier readout

Why Trans-Cleavage Efficiency Matters?

Upon crRNA-guided recognition of target dsDNA, Cas12a unleashes robust, non-specific collateral ssDNA trans-cleavage —the mechanism that drives signal amplification and underpins attomolar sensitivity in CRISPR diagnostics.

Target recognition→Cas12a activation→ Collateral cleavage of ssDNA reporter→ Amplified fluorescence signal

Higher trans-cleavage efficiency→ Faster signal accumulation→ Lower detection limit→ Earlier readout.

This is exactly why we engineered an optimized LbaCas12a.

 

Key Advantage

1. +57% Trans-Cleavage Activity, Lower Detection Limits

For single-copy and ultra-low-abundance targets, the 57% activity gain delivers a stronger signal window and greater headroom to push the limit of detection (LOD) lower. Higher activity also means a stronger signal within the same reaction time—or equivalent signal in less time—which is decisive for rapid readout in point-of-care (POCT) settings.

 

2. Faster Signal Response, Shorter Time-to-Result

The trans-cleavage boost translates directly into shorter assay times:

  • Fluorescence platforms: Faster time-to-positive(shorter time to detectable signal)
  • LFA - coupled formats：30-40min sample-to-result workflow.

 

3. High Purity, Lot-to-lot Consistency

  • Purity >95%（HPLC / SDS-PAGE verified)
  • Rigorous QC ensures lot-to-lot activity consistency — essential to IVD reagent development.


Performance Comparison


EZassay™ LbaCas12a

Commercial LbaCas12a

Fluorescence signal (identical conditions)

48300

30600


More than LbaCas12a—EZassay's CRISPR Enzyme Portfolio

 

EZassay offers the comprehensive Cas enzyme toolkit:

  • DNA Editing: AsCas12a, LbaCas12a (enhanced)
  • High-Temp & High-Specificity: AapCas12b, BrCas12b
  • RNA Detection: LwaCas13a (v2 enhanced), LbuCas13a
  • Miniature Cas: Cas14a (Un1Cas12f1), AsCas12f

From early-stage screening to assay validation, EZassay Biotech. accelerates your entire workflow with versatile solutions — ranging from bulk recombinant Cas proteins and cleavage reaction kits to end-to-end CRISPR detection kits.

Need Technical Support or Custom Formats?

Visit www.ezassay.com/en

Reach out at info@ezassay.com

#Lbacas12a #transcleavage #CRISPRDiagnostics #MolecularDiagnostics #LimitOfDetection #CasProteins #RPAassay