RCA

RCA Kits

High-yield isothermal amplification powered by an enhanced Phi29 polymerase, delivering sensitive, stable, and high-fidelity performance for advanced molecular analysis.

H01

Suitable for low-abundance targets

H02

Continuous amplification from circular DNA or RNA templates

H03

Compatible with sequencing, SNP analysis, and signal amplification applications

Product Overview

RCA is a high-yield isothermal amplification technology that uses circular DNA or RNA templates to generate long amplification products and repeated sequences, enabling sensitive detection and downstream molecular analysis.

  • Stable isothermal amplification with excellent sensitivity and reproducibility.
  • Continuous amplification driven by circular DNA or RNA templates.
  • Suitable for SNP analysis, signal amplification, and downstream analytical workflows.

Isothermal Amplification

Operates under mild reaction conditions with minimal temperature-control requirements, simplifying workflow standardization.

SNP Analysis

Compatible with probe-based detection strategies for SNP genotyping and mutation analysis.

POCT Ready

Results in as little as 20–40 minutes for rapid on-site testing.

Scalable Manufacturing

Stable formulations support large-scale production, long-term storage, and commercial deployment.

Comparison of Amplification Methods

A side-by-side comparison of common amplification workflows, key characteristics, and application boundaries to help you quickly choose the right methodology for your scenario.

ProductsRPALAMPRCAPCR
Temperature
37~42°C
60~65°C
30~42°C or room temperature
95°C-55°C-72°C cycling
Specificity
Sensitivity
Equipment
Simple device
Simple device
Not required
Precision instrument
Reaction Time
15~30 minutes
20~40 minutes
>4 hours
90~120 minutes
Cost
High
Low
Low
Low
Applications
  1. 1、CRISPR detection
  2. 2、Fluorescence assays
  3. 3、Lateral-flow testing
  1. 1、CRISPR detection
  2. 2、SNP analysis
  3. 3、Fluorescence and lateral-flow assays
  1. 1、Signal amplification
  2. 2、SNP analysis
  3. 3、Sequencing workflows
  1. 1、Clinical diagnostics
  2. 2、Forensic testing
  3. 3、Centralized laboratory analysis

1-on-1 Expert Support

Get personalized guidance from our technical experts to help solve complex research challenges, so you can stay focused on your science.

01

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Send us an email: info@ezassay.com

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