enzymes

Enzyme Raw Materials

Name: Enzyme Raw Materials
Brand: EZassay

Provides highly stable enzyme raw materials for nucleic-acid testing workflows, covering Cas proteins, LAMP, RCA, and common amplification enzymes from method development to scale-up.

H01

Batch-stable for R&D and mass production

H02

Covers multiple amplification and cleavage systems

H03

Rapid matching of enzyme solutions by scenario

Product List

Explore a broad range of enzymes for amplification, CRISPR detection, and molecular biology applications, supporting research, development, and manufacturing needs.

AsCas12a(Cpf1) Protein

A member of the Cas12a family of proteins. Derived from Acidaminococcus. It binds with crRNA to form functional complex, that specifically recognizes DNA sequences, and trans-cleaves ssDNA.

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LbaCas12a(Cpf1) Protein

A member of the Cas12a family of proteins. Derived from Lachnospiraceae. It binds with crRNA to form functional complex, that specifically recognizes DNA sequences, and trans-cleaves ssDNA.

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LbaCas12a(Cpf1) Protein (Enhanced v2)

Enhanced version of LbaCas12a. The efficiency is increased from 30% to 50%.

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LwaCas13a (C2c2) Protein (Enhanced v2)

Members of the Cas13a family of proteins, originating from Leptotrichia wadei, bind to crRNA, specifically recognize RNA sequences, and perform trans-cleavage of RNA. Compared to LbuCas13a, LwaCas13a exhibits high activity and cleaves RNA at a faster rate in certain diagnostic experiments.

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LbuCas13a (C2c2) Protein

Both LbuCas13a and LwaCas13a are RNA-targeting CRISPR enzymes from the Cas13 family. Compared to LwaCas13a, LbuCas13a exhibits stronger specificity. LbuCas13a is commonly used in diagnostic experiments requiring low background and high specificity, such as the detection of single-nucleotide variants (SNVs) and single-nucleotide polymorphisms (SNPs).

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Cas14a (Cas12f1) Protein

Cas14a is a very small Cas protein, about half that of other Cas proteins. Cas14a can recognize and cleave the target ssDNA, and also has co-cleavage activity. It can be used for the detection of ssDNA. Cas14 enables high-fidelity SNP genotyping. Since the recognition of DNA molecules does not depend on the PAM site, Cas14 has extremely strict requirements for the accuracy of the recognition sequence, and the mismatch of a single base will seriously inhibit its cleavage activity.

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AapCas12b Protein

A member of the Cas12b family of proteins. Derived from Alicyclobacillus acidiphilus. With thermostable character, it can be used with LAMP.

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BrCas12b Protein

A member of the Cas12b family of proteins. Derived from Brevibacillus leverages. With thermostable character, it can be used with LAMP.

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dCas9_NLS Protein

dCas9-NLS protein

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EiCsm6 Protein

It can be activated by cleavage products of Cas13a or Cas13b, effectively enhancing the signal intensity of CRISPR detection.

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Selected Publications

A collection of representative studies using this kit, highlighting its applications across diverse research areas.

总文献数: 31最高 IF: 22.5

Zhang Y et al. Detection of biological loads in sewage using the automated robot-driven photoelectrochemical biosensing platform. Exploration (Beijing). 2024 Mar 14. Impact Factor: 22.5
Wang, J., et al. Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b. Nat Commun. 16, 6392 (2025). Impact factor:15.7
Jingfu Wang, et al.Amplification-free detection of Porphyromonas gingivalis with no-PAM CRISPR-Cas12a using a smartphone-based platform. Chemical Engineering Journal. Volume 510,2025. Impact Factor: 13.3
Huang W, et al. Expanding Cas12a Activity Control with an RNA G-Quadruplex at the 5' end of CRISPR RNA. Adv Sci (Weinh). 2025 Feb. Impact Factor: 14.9
Huang W,. Expanding Cas12a Activity Control with an RNA G-Quadruplex at the 5' end of CRISPR RNA. Adv Sci (Weinh). 2025 Feb;12(7):e2411305. Impact factor: 14.3
Jiayu Feng, et.al. A novel thermo-activated one-pot RPA-CRISPR-Cas12b assay for Mycoplasma pneumoniae POCT. Biosensors and Bioelectronics.Volume 288,2025. Impact factor:12.6
Shen J, et al. CRISPR/Cas12a-Assisted isothermal amplification for rapid and specific diagnosis of respiratory virus on an microfluidic platform. Biosens Bioelectron. 2023 Oct 1. Impact factor:11.2
Zeng Q, et al. Sensitive and visual detection of SARS-CoV-2 using RPA-Cas12a one-step assay with ssDNA-modified crRNA. Anal Chim Acta. 2024 Jun 22. Impact factor:11.2
Zhu, Wenhui, et al. One-Pot Photocontrolled CRISPR-Cas12b Coupled with Loop-Mediated Isothermal Amplification Assay for Point-of-Care Test of Mycoplasma pneumonia. ACS sensors. 2025. Impact Factor: 9.1
Xie R, et al. Rapid Bacterial Identification through Multiplexed Nucleic Acid Detection on a Digital Microfluidic Platform for Enhanced Clinical Intervention against Infections. ACS Sens. 2025 Feb 10. Impact factor:8.2